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BCC199. These data might be a result of the potent microplate-based cultivation method made use of During this review. This method may perhaps not simply quantitatively identify the quantity of certain enzyme things to do and also do away with fungal isolates that do not mature very well below submerged fermentation conditions.
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A miniaturized society process that scales down the fermentation quantity into a 24-well microtiter plate has been claimed to successfully lessen labor and aid experiments with a large number of fungal strains [26]. This technique was productively demonstrated in solid cultivation of Trichoderma
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0.five.three [36] and run through an ordinary nucleotide BLAST (Primary Community Alignment Research Instrument; NCBI nucleotide databases; ) To guage the similarity with documented sequences more info of fungal species. Fungal sequences had been then analyzed by comparison with the sort species sequences. All new fungal sequences have been deposited inside the NCBI databases beneath the accession quantities proven in Desk S1.
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